NRF1 ELISA Kit

Produktnummer ABIN5657466

Produktdetails NRF1 ELISA Kit

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Nuclear Respiratory Factor 1 (NRF1). No significant cross-reactivity or interference between Nuclear Respiratory Factor 1 (NRF1) and analogues was observed.

Sensitivität: 28 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Respiratory Factor 1 (NRF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Respiratory Factor 1 (NRF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Respiratory Factor 1 (NRF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Respiratory Factor 1 (NRF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Respiratory Factor 1 (NRF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Respiratory Factor 1 (NRF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Andere Bezeichnung: Nuclear Respiratory Factor 1 (NRF1 Produkte)

Synonyme: ALPHA-PAL ELISA Kit, C87038 ELISA Kit, D6Ertd415e ELISA Kit, SC:BZ3C13.1 ELISA Kit, SC:bZ3C13.2 ELISA Kit, ube2h ELISA Kit, Nfe2l1_retired ELISA Kit, IBR/F ELISA Kit, NRF1 ELISA Kit, alpha-pal ELISA Kit, nrf-1 ELISA Kit, LOC100220087 ELISA Kit, nuclear respiratory factor 1 ELISA Kit, nuclear respiratory factor 1 S homeolog ELISA Kit, NRF1 ELISA Kit, Nrf1 ELISA Kit, nrf1 ELISA Kit, nrf1.S ELISA Kit

Hintergrund:

Gene Name: Nuclear Respiratory Factor 1

Gene Aliases: ALPHA-PAL, EWG, Alpha Palindromic-Binding Protein

Pathways: Regulation of Lipid Metabolism by PPARalpha