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OBP2A ELISA Kit

OBP2A Reaktivität: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Saliva, Tissue Homogenate
Produktnummer ABIN5657494
  • Target Alle OBP2A ELISA Kits anzeigen
    OBP2A (Odorant Binding Protein 2A (OBP2A))
    Reaktivität
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.781 ng/mL - 50 ng/mL
    Untere Nachweisgrenze
    0.781 ng/mL
    Applikation
    ELISA
    Proben
    Saliva, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Odorant Binding Protein 2A (OBP2A). No significant cross-reactivity or interference between Odorant Binding Protein 2A (OBP2A) and analogues was observed.
    Sensitivität
    0.31 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Odorant Binding Protein 2A (OBP2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Odorant Binding Protein 2A (OBP2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Odorant Binding Protein 2A (OBP2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Odorant Binding Protein 2A (OBP2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Odorant Binding Protein 2A (OBP2A) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Odorant Binding Protein 2A (OBP2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle OBP2A ELISA Kits anzeigen
    OBP2A (Odorant Binding Protein 2A (OBP2A))
    Andere Bezeichnung
    Odorant Binding Protein 2A (OBP2A Produkte)
    Synonyme
    OBP ELISA Kit, OBP2C ELISA Kit, OBPIIa ELISA Kit, hOBPIIa ELISA Kit, BC027556 ELISA Kit, Lcn13 ELISA Kit, odorant binding protein 2A ELISA Kit, OBP2A ELISA Kit, Obp2a ELISA Kit
    Hintergrund

    Gene Name: Odorant Binding Protein 2A

    Gene Aliases: OBP2-A, OBP, OBP2C, OBPIIa, hOBPIIa

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