SP7 ELISA Kit

Produktnummer ABIN5657565

Produktdetails SP7 ELISA Kit

Target: SP7 (Sp7 Transcription Factor (SP7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Osterix (OSX). No significant cross-reactivity or interference between Osterix (OSX) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osterix (OSX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osterix (OSX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osterix (OSX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osterix (OSX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osterix (OSX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osterix (OSX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SP7

Target: SP7 (Sp7 Transcription Factor (SP7))

Andere Bezeichnung: Osterix (SP7 Produkte)

Synonyme: BBBG1 ELISA Kit, FXY ELISA Kit, GBBB1 ELISA Kit, MIDIN ELISA Kit, OGS1 ELISA Kit, OS ELISA Kit, OSX ELISA Kit, RNF59 ELISA Kit, TRIM18 ELISA Kit, XPRF ELISA Kit, ZNFXY ELISA Kit, OI11 ELISA Kit, OI12 ELISA Kit, osterix ELISA Kit, Osx ELISA Kit, osx ELISA Kit, SP7 ELISA Kit, sp7 ELISA Kit, 6430578P22Rik ELISA Kit, C22 ELISA Kit, midline 1 ELISA Kit, Sp7 transcription factor ELISA Kit, uncharacterized LOC100063836 ELISA Kit, Sp7 transcription factor L homeolog ELISA Kit, Sp7 transcription factor 7 ELISA Kit, MID1 ELISA Kit, SP7 ELISA Kit, Sp7 ELISA Kit, sp7 ELISA Kit, LOC100063836 ELISA Kit, sp7.L ELISA Kit

Hintergrund:

Gene Name: Osterix

Gene Aliases: Sp7 Transcription Factor, Specificity Protein 7, Zinc finger protein osterix