Peroxiredoxin 5 ELISA Kit

Produktnummer ABIN5657728

Produktdetails Peroxiredoxin 5 ELISA Kit

Target: Peroxiredoxin 5 (PRDX5)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Cerebrospinal Fluid, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 5 (PRDX5). No significant cross-reactivity or interference between Peroxiredoxin 5 (PRDX5) and analogues was observed.

Sensitivität: 12.8 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 5 (PRDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 5 (PRDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 5 (PRDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 5 (PRDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 5 (PRDX5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 5 (PRDX5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Peroxiredoxin 5

Target: Peroxiredoxin 5 (PRDX5)

Andere Bezeichnung: Peroxiredoxin 5 (PRDX5 Produkte)

Synonyme: acr1 ELISA Kit, aoeb166 ELISA Kit, pmp20 ELISA Kit, prx-5 ELISA Kit, prx5 ELISA Kit, prxv ELISA Kit, sbbi10 ELISA Kit, GB16965 ELISA Kit, zgc:112318 ELISA Kit, PRDX5 ELISA Kit, CG 32920 ELISA Kit, CG 7217 ELISA Kit, CG32920 ELISA Kit, CG7217 ELISA Kit, Dmel\\CG7217 ELISA Kit, dPrx5 ELISA Kit, dprx5 ELISA Kit, ACR1 ELISA Kit, AOEB166 ELISA Kit, B166 ELISA Kit, PLP ELISA Kit, PMP20 ELISA Kit, PRDX6 ELISA Kit, PRXV ELISA Kit, prx-V ELISA Kit, AOPP ELISA Kit, Pmp20 ELISA Kit, Prdx6 ELISA Kit, PrxV ELISA Kit, Aoeb166 ELISA Kit, Prx V ELISA Kit, peroxiredoxin 5 L homeolog ELISA Kit, peroxiredoxin-5, mitochondrial ELISA Kit, peroxiredoxin 5 ELISA Kit, Peroxiredoxin 5 ELISA Kit, prdx5.L ELISA Kit, LOC552429 ELISA Kit, prdx5 ELISA Kit, PRDX5 ELISA Kit, Prx5 ELISA Kit, Prdx5 ELISA Kit

Hintergrund:

Gene Name: Peroxiredoxin 5

Gene Aliases: PLP, PRDX6, ACR1, PMP20, PRXV, SBBI10, Thioredoxin Peroxidase PMP20, Peroxisomal Antioxidant Enzyme, TPx Type VI, Liver Tissue 2D-Page Spot 71B, Alu Co-Repressor 1

Gen-ID: 25824

UniProt: P30044

Pathways: Cell RedoxHomeostasis