PPARD ELISA Kit

Produktnummer ABIN5657735

Produktdetails PPARD ELISA Kit

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Delta (PPARd). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Delta (PPARd) and analogues was observed.

Sensitivität: 0.115 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Delta (PPARd). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Delta (PPARd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Delta (PPARd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Delta (PPARd) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Delta (PPARd) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PPARD

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Andere Bezeichnung: Peroxisome Proliferator Activated Receptor Delta (PPARD Produkte)

Synonyme: FAAR ELISA Kit, NR1C2 ELISA Kit, NUC1 ELISA Kit, NUCI ELISA Kit, NUCII ELISA Kit, PPARB ELISA Kit, NUC-1 ELISA Kit, Nr1c2 ELISA Kit, PPAR-beta ELISA Kit, PPAR-delta ELISA Kit, PPAR[b] ELISA Kit, PPARdelta ELISA Kit, Pparb ELISA Kit, Pparb/d ELISA Kit, PPARBETA ELISA Kit, ppard ELISA Kit, pparb ELISA Kit, PPARD ELISA Kit, PPAR-D/B ELISA Kit, peroxisome proliferator activated receptor delta ELISA Kit, peroxisome proliferator activator receptor delta ELISA Kit, peroxisome proliferator activated receptor delta S homeolog ELISA Kit, peroxisome proliferator-activated receptor delta ELISA Kit, PPARD ELISA Kit, Ppard ELISA Kit, ppard.S ELISA Kit, ppard ELISA Kit

Hintergrund:

Gene Name: Peroxisome Proliferator Activated Receptor Delta

Gene Aliases: FAAR, NR1C2, NUC1, NUCI, PPARB, PPARd/B, Nuclear Receptor Subfamily 1,Group C,Member 2, Nuclear hormone receptor 1, Peroxisome proliferator-activated receptor beta

Gen-ID: 5467

UniProt: Q03181

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation