PFKM ELISA Kit

Produktnummer ABIN5657776

Produktdetails PFKM ELISA Kit

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Phosphofructokinase, Muscle (PFKM). No significant cross-reactivity or interference between Phosphofructokinase, Muscle (PFKM) and analogues was observed.

Sensitivität: 0.112 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphofructokinase, Muscle (PFKM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphofructokinase, Muscle (PFKM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphofructokinase, Muscle (PFKM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphofructokinase, Muscle (PFKM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphofructokinase, Muscle (PFKM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphofructokinase, Muscle (PFKM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Andere Bezeichnung: Phosphofructokinase, Muscle (PFKM Produkte)

Synonyme: GSD7 ELISA Kit, PFK-1 ELISA Kit, PFK1 ELISA Kit, PFKA ELISA Kit, PFKX ELISA Kit, AI131669 ELISA Kit, PFK-A ELISA Kit, PFK-M ELISA Kit, Pfk-4 ELISA Kit, Pfk4 ELISA Kit, Pfka ELISA Kit, Pfkx ELISA Kit, Pfk-M ELISA Kit, M-PFK ELISA Kit, PFKM ELISA Kit, pfkm ELISA Kit, zgc:92344 ELISA Kit, pfk ELISA Kit, im:7137110 ELISA Kit, wu:fc58g11 ELISA Kit, phosphofructokinase, muscle ELISA Kit, phosphofructokinase, platelet ELISA Kit, phosphofructokinase, muscle a ELISA Kit, phosphofructokinase, muscle S homeolog ELISA Kit, phosphofructokinase, muscle b ELISA Kit, PFKM ELISA Kit, Pfkm ELISA Kit, PFKP ELISA Kit, pfkma ELISA Kit, pfkm.S ELISA Kit, pfkmb ELISA Kit

Hintergrund:

Gene Name: Phosphofructokinase, Muscle

Gene Aliases: PFK1, PFKX, Phosphofructokinase 1, Phosphofructo-1-kinase isozyme A, 6-Phosphofructokinase Polypeptide X, Fructose-6-Phosphate 1-Phosphotransferase

Gen-ID: 65152

UniProt: P47858

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effekt