PNPLA2 ELISA Kit

Produktnummer ABIN5657791

Produktdetails PNPLA2 ELISA Kit

Target: PNPLA2 (Calcium Independent Phospholipase A2 (PNPLA2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Calcium Independent (iPLA2). No significant cross-reactivity or interference between Phospholipase A2, Calcium Independent (iPLA2) and analogues was observed.

Sensitivität: 0.129 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Calcium Independent (iPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Calcium Independent (iPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Calcium Independent (iPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Calcium Independent (iPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Calcium Independent (iPLA2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Calcium Independent (iPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PNPLA2

Target: PNPLA2 (Calcium Independent Phospholipase A2 (PNPLA2))

Andere Bezeichnung: Phospholipase A2, Calcium Independent (PNPLA2 Produkte)

Synonyme: 1110001C14Rik ELISA Kit, ATGL ELISA Kit, PEDF-R ELISA Kit, TTS-2.2 ELISA Kit, TTS2 ELISA Kit, iPLA2zeta ELISA Kit, BB112799 ELISA Kit, PNPLA9 ELISA Kit, iPLA(2)beta ELISA Kit, iPLA2 ELISA Kit, iPLA2beta ELISA Kit, patatin like phospholipase domain containing 2 ELISA Kit, phospholipase A2, group VI ELISA Kit, PNPLA2 ELISA Kit, Pla2g6 ELISA Kit

Hintergrund:

Gene Name: Phospholipase A2, Calcium Independent

Gene Aliases: PLA2G6, CaI-PLA2, GVI, INAD1, PNPLA9, Ca2+ Independent PLA2, 85 kDa Calcium-Independent Phospholipase A2, Phospholipase A2,group VI(Cytosolic,Calcium-Independent)

Gen-ID: 360426

UniProt: P97570