PSPH CLIA Kit

Produktnummer ABIN5657820

Produktdetails PSPH CLIA Kit

Target: PSPH (Phosphoserine Phosphatase (PSPH))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Phosphoserine Phosphatase (PSPH). No significant cross-reactivity or interference between Phosphoserine Phosphatase (PSPH) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Phosphoserine Phosphatase (PSPH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Phosphoserine Phosphatase (PSPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Phosphoserine Phosphatase (PSPH) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoserine Phosphatase (PSPH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoserine Phosphatase (PSPH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: PSPH (Phosphoserine Phosphatase (PSPH))

Andere Bezeichnung: Phosphoserine Phosphatase (PSPH Produkte)

Synonyme: PSP CLIA Kit, PSPHD CLIA Kit, AI480570 CLIA Kit, PSPase CLIA Kit, PSPH CLIA Kit, psph CLIA Kit, MGC78828 CLIA Kit, zgc:112414 CLIA Kit, DDBDRAFT_0183847 CLIA Kit, DDBDRAFT_0230054 CLIA Kit, DDB_0183847 CLIA Kit, DDB_0230054 CLIA Kit, phosphoserine phosphatase CLIA Kit, phosphoserine phosphatase L homeolog CLIA Kit, PSP; O-phosphoserine phosphohydrolase; PSPase CLIA Kit, phosphoserine phosphatase SerB CLIA Kit, BPI fold containing family A member 2 CLIA Kit, PSPH CLIA Kit, Psph CLIA Kit, psph.L CLIA Kit, psph CLIA Kit, serB CLIA Kit, serB-1 CLIA Kit, LOC5568588 CLIA Kit, PSP CLIA Kit, Svir_29300 CLIA Kit, MMAH_RS03435 CLIA Kit, BPIFA2 CLIA Kit

Hintergrund:

Gene Name: Phosphoserine Phosphatase

Gene Aliases: PSP, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase

Pathways: Warburg Effekt