PDGFC ELISA Kit
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- Target Alle PDGFC ELISA Kits anzeigen
- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 125.00 pg/mL - 8000 pg/mL
- Untere Nachweisgrenze
- 125.00 pg/mL
- Applikation
- ELISA
- Proben
- Plasma
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor C (PDGFC). No significant cross-reactivity or interference between Platelet Derived Growth Factor C (PDGFC) and analogues was observed.
- Sensitivität
- 43 pg/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor C (PDGFC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor C (PDGFC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor C (PDGFC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor C (PDGFC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor C (PDGFC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor C (PDGFC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle PDGFC ELISA Kits anzeigen
- PDGFC (Platelet-Derived Growth Factor C (PDGFC))
- Andere Bezeichnung
- Platelet Derived Growth Factor C (PDGFC Produkte)
- Synonyme
- SCDGF ELISA Kit, FALLOTEIN ELISA Kit, 1110064L01Rik ELISA Kit, AI647969 ELISA Kit, PDGF-C ELISA Kit, platelet derived growth factor C ELISA Kit, platelet-derived growth factor, C polypeptide ELISA Kit, PDGFC ELISA Kit, Pdgfc ELISA Kit
- Substanzklasse
- Viral Protein
- Hintergrund
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Gene Name: Platelet Derived Growth Factor C
Gene Aliases: PDGF-C, SCDGF, VEGF-E, Fallotein, Spinal cord-derived growth factor, Platelet-derived growth factor C, latent form, Platelet-derived growth factor C, receptor-binding form
- Gen-ID
- 56034
- UniProt
- Q9NRA1
- Pathways
- RTK Signalweg, Platelet-derived growth Factor Receptor Signaling
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