PVRL1 ELISA Kit

Produktnummer ABIN5657967

Produktdetails PVRL1 ELISA Kit

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Poliovirus Receptor Related Protein 1 (PVRL1). No significant cross-reactivity or interference between Poliovirus Receptor Related Protein 1 (PVRL1) and analogues was observed.

Sensitivität: 0.063 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Poliovirus Receptor Related Protein 1 (PVRL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Poliovirus Receptor Related Protein 1 (PVRL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Poliovirus Receptor Related Protein 1 (PVRL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Poliovirus Receptor Related Protein 1 (PVRL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PVRL1

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Andere Bezeichnung: Poliovirus Receptor Related Protein 1 (PVRL1 Produkte)

Synonyme: CD111 ELISA Kit, CLPED1 ELISA Kit, ED4 ELISA Kit, HIgR ELISA Kit, HVEC ELISA Kit, OFC7 ELISA Kit, PRR ELISA Kit, PRR1 ELISA Kit, PVRR ELISA Kit, PVRR1 ELISA Kit, SK-12 ELISA Kit, nectin-1 ELISA Kit, prr1 ELISA Kit, PVRL1 ELISA Kit, pvrl1 ELISA Kit, AI835281 ELISA Kit, AW549174 ELISA Kit, Cd111 ELISA Kit, HveC ELISA Kit, cd111 ELISA Kit, cd112 ELISA Kit, clped1 ELISA Kit, ed4 ELISA Kit, higr ELISA Kit, hveb ELISA Kit, hvec ELISA Kit, nectin-2 ELISA Kit, ofc7 ELISA Kit, prr ELISA Kit, prr2 ELISA Kit, pvrl2 ELISA Kit, pvrl2.L ELISA Kit, pvrr ELISA Kit, pvrr1 ELISA Kit, pvrr2 ELISA Kit, sk-12 ELISA Kit, nectin cell adhesion molecule 1 ELISA Kit, nectin-1 ELISA Kit, Poliovirus receptor-related protein 1 ELISA Kit, poliovirus receptor-related 1 (herpesvirus entry mediator C) ELISA Kit, nectin cell adhesion molecule 2 L homeolog ELISA Kit, NECTIN1 ELISA Kit, LOC100145553 ELISA Kit, pvrl1 ELISA Kit, nectin1 ELISA Kit, Nectin1 ELISA Kit, PVRL1 ELISA Kit, nectin2.L ELISA Kit

Hintergrund:

Gene Name: Poliovirus Receptor Related Protein 1

Gene Aliases: CD111, HVEC, PRR1, CLPED1, ED4, HIgR, OFC7, PRR, PVRR1, SK12, Nectin 1, Herpesvirus Entry Mediator C, Herpesvirus Ig-like receptor

Pathways: Cell-Cell Junction Organization