PFN1 ELISA Kit

Produktnummer ABIN5658062

Produktdetails PFN1 ELISA Kit

Target: PFN1 (Profilin 1 (PFN1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Profilin 1 (PFN1). No significant cross-reactivity or interference between Profilin 1 (PFN1) and analogues was observed.

Sensitivität: 30 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Profilin 1 (PFN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Profilin 1 (PFN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Profilin 1 (PFN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Profilin 1 (PFN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Profilin 1 (PFN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Profilin 1 (PFN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PFN1

Target: PFN1 (Profilin 1 (PFN1))

Andere Bezeichnung: Profilin 1 (PFN1 Produkte)

Synonyme: GmPRO1 ELISA Kit, GmPRO2 ELISA Kit, PRO1 ELISA Kit, Profilin-1 ELISA Kit, profilin-1 ELISA Kit, profilin ELISA Kit, MGC130883 ELISA Kit, Profilin1 ELISA Kit, XProfilin1 ELISA Kit, sb:cb813 ELISA Kit, wu:fa91a03 ELISA Kit, ALS18 ELISA Kit, Pfn ELISA Kit, F6F22.21 ELISA Kit, F6F22_21 ELISA Kit, PFN1 ELISA Kit, PROFILIN 1 ELISA Kit, profilin 1 ELISA Kit, profilin ELISA Kit, profilin 1 ELISA Kit, profilin 1 L homeolog ELISA Kit, profilin homolog 1 ELISA Kit, Profilin-1 ELISA Kit, PRO2 ELISA Kit, PFN1 ELISA Kit, pfn1.L ELISA Kit, pfn1 ELISA Kit, Pfn1 ELISA Kit, PRF1 ELISA Kit, prf1 ELISA Kit, pfn-1 ELISA Kit

Hintergrund:

Gene Name: Profilin 1

Gene Aliases: Epididymis tissue protein Li 184a

Gen-ID: 5216

UniProt: P07737

Pathways: Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location