PSMA7 ELISA Kit

Produktnummer ABIN5658183

Produktdetails PSMA7 ELISA Kit

Target: PSMA7 (Proteasome Subunit alpha 7 (PSMA7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Alpha Type 7 (PSMa7). No significant cross-reactivity or interference between Proteasome Subunit Alpha Type 7 (PSMa7) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 7 (PSMa7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 7 (PSMa7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 7 (PSMa7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proteasome Subunit Alpha Type 7 (PSMa7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 7 (PSMa7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 7 (PSMa7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PSMA7

Target: PSMA7 (Proteasome Subunit alpha 7 (PSMA7))

Andere Bezeichnung: Proteasome Subunit Alpha Type 7 (PSMA7 Produkte)

Synonyme: C6 ELISA Kit, HSPC ELISA Kit, RC6-1 ELISA Kit, XAPC7 ELISA Kit, C6-I ELISA Kit, DDBDRAFT_0185059 ELISA Kit, DDBDRAFT_0217080 ELISA Kit, DDB_0185059 ELISA Kit, DDB_0217080 ELISA Kit, hspc ELISA Kit, rc6-1 ELISA Kit, xapc7 ELISA Kit, psma7 ELISA Kit, psma4-B ELISA Kit, proteasome subunit alpha 7 ELISA Kit, proteasome (prosome, macropain) subunit, alpha type 7 ELISA Kit, 20S proteasome subunit alpha-7 ELISA Kit, proteasome subunit alpha 7 S homeolog ELISA Kit, proteasome subunit alpha 7 L homeolog ELISA Kit, PSMA7 ELISA Kit, Psma7 ELISA Kit, psmA7 ELISA Kit, psma7 ELISA Kit, psma7.S ELISA Kit, psma7.L ELISA Kit

Hintergrund:

Gene Name: Proteasome Subunit Alpha Type 7

Gene Aliases: C6, HSPC, RC6-1, XAPC7

Gen-ID: 5688

UniProt: O14818

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA