P4HB ELISA Kit

Produktnummer ABIN5658197

Produktdetails P4HB ELISA Kit

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase (PDI). No significant cross-reactivity or interference between Protein Disulfide Isomerase (PDI) and analogues was observed.

Sensitivität: 0.114 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase (PDI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase (PDI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase (PDI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase (PDI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Andere Bezeichnung: Protein Disulfide Isomerase (P4HB Produkte)

Synonyme: DSI ELISA Kit, ERBA2L ELISA Kit, GIT ELISA Kit, P4Hbeta ELISA Kit, PDI ELISA Kit, PDIA1 ELISA Kit, PHDB ELISA Kit, PO4DB ELISA Kit, PO4HB ELISA Kit, PROHB ELISA Kit, PDA2 ELISA Kit, PDIP ELISA Kit, PDIR ELISA Kit, ERp59 ELISA Kit, Pdia1 ELISA Kit, Thbp ELISA Kit, p55 ELISA Kit, XPDIp ELISA Kit, pdi ELISA Kit, 1810041F13Rik ELISA Kit, AI661267 ELISA Kit, Pdip ELISA Kit, Pdipl ELISA Kit, p58 ELISA Kit, prolyl 4-hydroxylase subunit beta ELISA Kit, protein disulfide isomerase family A member 2 ELISA Kit, prolyl 4-hydroxylase, beta polypeptide ELISA Kit, protein disulfide isomerase family A member 2 L homeolog ELISA Kit, protein disulfide isomerase associated 2 ELISA Kit, prolyl 4-hydroxylase subunit beta L homeolog ELISA Kit, protein disulfide-isomerase ELISA Kit, P4HB ELISA Kit, PDIA2 ELISA Kit, P4hb ELISA Kit, pdia2.L ELISA Kit, Pdia2 ELISA Kit, p4hb.L ELISA Kit, LOC8281065 ELISA Kit

Hintergrund:

Gene Name: Protein Disulfide Isomerase

Gene Aliases: P4HB, ERBA2L, PDIA1, PO4DB, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta, Prolyl 4-Hydroxylase Subunit Beta, Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

Gen-ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism