AKT2 ELISA Kit

Produktnummer ABIN5658211

Produktdetails AKT2 ELISA Kit

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Kinase B Beta (PKBb). No significant cross-reactivity or interference between Protein Kinase B Beta (PKBb) and analogues was observed.

Sensitivität: 0.124 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Beta (PKBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Beta (PKBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Beta (PKBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Beta (PKBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase B Beta (PKBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase B Beta (PKBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Andere Bezeichnung: Protein Kinase B Beta (AKT2 Produkte)

Synonyme: AKT2 ELISA Kit, pkbb ELISA Kit, pkbbeta ELISA Kit, pkbss ELISA Kit, prkbb ELISA Kit, rac-beta ELISA Kit, v-akt2 ELISA Kit, 2410016A19Rik ELISA Kit, AW554154 ELISA Kit, PKB ELISA Kit, PKBbeta ELISA Kit, HIHGHH ELISA Kit, PKBB ELISA Kit, PKBBETA ELISA Kit, PRKBB ELISA Kit, RAC-BETA ELISA Kit, cb945 ELISA Kit, akt2-a ELISA Kit, AKT serine/threonine kinase 2 ELISA Kit, v-akt murine thymoma viral oncogene homolog 2 ELISA Kit, thymoma viral proto-oncogene 2 ELISA Kit, v-akt murine thymoma viral oncogene homolog 2 L homeolog ELISA Kit, AKT2 ELISA Kit, akt2 ELISA Kit, Akt2 ELISA Kit, akt2.L ELISA Kit

Hintergrund:

Gene Name: Protein Kinase B Beta

Gene Aliases: AKT2, PKBBETA, PRKB-B, RAC-BETA, RAC-Beta Serine/Threonine-Protein Kinase, V-Akt Murine Thymoma Viral Oncogene Homolog 2

Gen-ID: 208

UniProt: P31751

Pathways: PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, TLR Signalweg, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling