EIF2AK2 ELISA Kit

Produktnummer ABIN5658229

Produktdetails EIF2AK2 ELISA Kit

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Kinase R (PKR). No significant cross-reactivity or interference between Protein Kinase R (PKR) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase R (PKR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase R (PKR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase R (PKR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase R (PKR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase R (PKR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase R (PKR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Andere Bezeichnung: Protein Kinase R (EIF2AK2 Produkte)

Synonyme: PKR ELISA Kit, pkr ELISA Kit, EIF2AK2 ELISA Kit, DKFZp469K1934 ELISA Kit, EIF2AK1 ELISA Kit, PRKR ELISA Kit, 2310047A08Rik ELISA Kit, 4732414G15Rik ELISA Kit, AI467567 ELISA Kit, AI747578 ELISA Kit, Pkr ELISA Kit, Prkr ELISA Kit, Tik ELISA Kit, eukaryotic translation initiation factor 2 alpha kinase 2 ELISA Kit, eukaryotic translation initiation factor 2-alpha kinase 2 ELISA Kit, EIF2AK2 ELISA Kit, eif2ak2 ELISA Kit, Eif2ak2 ELISA Kit

Hintergrund:

Gene Name: Protein Kinase R

Gene Aliases: PRKR, PRK-R, EIF2AK2, EIF2AK1, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, P1/eIF-2A kinase

Gen-ID: 5610

UniProt: P19525

Pathways: DNA Reparatur, ER-Nucleus Signaling, Hepatitis C