PRKDC ELISA Kit

Produktnummer ABIN5658240

Produktdetails PRKDC ELISA Kit

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). No significant cross-reactivity or interference between Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) and analogues was observed.

Sensitivität: 0.105 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Andere Bezeichnung: Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC Produkte)

Synonyme: DNA-PKcs ELISA Kit, DNAPK ELISA Kit, DNPK1 ELISA Kit, HYRC ELISA Kit, HYRC1 ELISA Kit, XRCC7 ELISA Kit, p350 ELISA Kit, DNA-PKCS ELISA Kit, dna-pkcs ELISA Kit, dnapk ELISA Kit, dnpk1 ELISA Kit, hyrc ELISA Kit, hyrc1 ELISA Kit, prkdc-A ELISA Kit, xrcc7 ELISA Kit, PRKDC ELISA Kit, AI326420 ELISA Kit, AU019811 ELISA Kit, DNAPDcs ELISA Kit, DOXNPH ELISA Kit, dxnph ELISA Kit, p460 ELISA Kit, scid ELISA Kit, slip ELISA Kit, protein kinase, DNA-activated, catalytic polypeptide ELISA Kit, protein kinase, DNA-activated, catalytic polypeptide L homeolog ELISA Kit, DNA-dependent protein kinase catalytic subunit ELISA Kit, protein kinase, DNA activated, catalytic polypeptide ELISA Kit, PRKDC ELISA Kit, prkdc.L ELISA Kit, LOC100633457 ELISA Kit, Prkdc ELISA Kit

Hintergrund:

Gene Name: Protein Kinase, DNA Activated, Catalytic Polypeptide

Gene Aliases: HYRC, HYRC1, DNPK1, p350, DNAPK, XRCC7, DNA-PKcs

Gen-ID: 19090

UniProt: P97313

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response