PKLR ELISA Kit

Produktnummer ABIN5658320

Produktdetails PKLR ELISA Kit

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 39.06 pg/mL - 2500 pg/mL

Untere Nachweisgrenze: 39.06 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Pyruvate Kinase, Liver And RBC (PK). No significant cross-reactivity or interference between Pyruvate Kinase, Liver And RBC (PK) and analogues was observed.

Sensitivität: 17 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Liver And RBC (PK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Liver And RBC (PK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Liver And RBC (PK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Liver And RBC (PK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Kinase, Liver And RBC (PK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Kinase, Liver And RBC (PK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Andere Bezeichnung: Pyruvate Kinase, Liver And RBC (PKLR Produkte)

Synonyme: PK1 ELISA Kit, PKL ELISA Kit, PKR ELISA Kit, PKRL ELISA Kit, RPK ELISA Kit, Pklg ELISA Kit, wu:fd15e01 ELISA Kit, wu:fi37e08 ELISA Kit, pk1 ELISA Kit, PKLR ELISA Kit, Pk-1 ELISA Kit, Pk1 ELISA Kit, R-PK ELISA Kit, pklr ELISA Kit, pyruvate kinase L/R ELISA Kit, pyruvate kinase, liver and RBC ELISA Kit, pyruvate kinase, liver and RBC L homeolog ELISA Kit, pyruvate kinase liver and red blood cell ELISA Kit, pyruvate kinase PKLR-like ELISA Kit, PKLR ELISA Kit, Pklr ELISA Kit, pklr ELISA Kit, pklr.L ELISA Kit, LOC100621940 ELISA Kit

Hintergrund:

Gene Name: Pyruvate Kinase, Liver And RBC

Gene Aliases: PKLR, PyK, PK1, PKL, Pyruvate Kinase Isozymes R/L, Glutamic--pyruvic transaminase 1, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

Gen-ID: 5313

UniProt: P30613

Pathways: Ribonucleoside Biosynthetic Process