QSOX1 ELISA Kit

Produktnummer ABIN5658326

Produktdetails QSOX1 ELISA Kit

Target: QSOX1 (Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). No significant cross-reactivity or interference between Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) and analogues was observed.

Sensitivität: 30 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu QSOX1

Target: QSOX1 (Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1))

Andere Bezeichnung: Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1 Produkte)

Synonyme: qscn6l1 ELISA Kit, Qscn6 ELISA Kit, Qsox ELISA Kit, Sox-2 ELISA Kit, rSOx ELISA Kit, Q6 ELISA Kit, QSCN6 ELISA Kit, 1300003H02Rik ELISA Kit, QSOX ELISA Kit, SOx ELISA Kit, GEC-3 ELISA Kit, GEC3 ELISA Kit, SOX3 ELISA Kit, ATQSOX1 ELISA Kit, QSO2 ELISA Kit, T15D22.7 ELISA Kit, T15D22_7 ELISA Kit, quiescin-sulfhydryl oxidase 1 ELISA Kit, quiescin sulfhydryl oxidase 1 ELISA Kit, quiescin sulfhydryl oxidase 1 L homeolog ELISA Kit, quiescin Q6 sulfhydryl oxidase 1 ELISA Kit, quiescin-sulfhydryl oxidase 1 ELISA Kit, QSOX1 ELISA Kit, qsox1 ELISA Kit, qsox1.L ELISA Kit, Qsox1 ELISA Kit

Hintergrund:

Gene Name: Quiescin Q6 Sulfhydryl Oxidase 1

Gene Aliases: Q6, QSCN6

Gen-ID: 104009

UniProt: Q8BND5

Pathways: Cell RedoxHomeostasis