RAB5 CLIA Kit

Produktnummer ABIN5658340

Produktdetails RAB5 CLIA Kit

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.594 ng/mL - 38 ng/mL

Untere Nachweisgrenze: 0.594 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A). No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.

Sensitivität: 0.02 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the RAB5A, Member RAS Oncogene Family (RAB5A) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Andere Bezeichnung: RAB5A, Member RAS Oncogene Family (RAB5A Produkte)

Synonyme: RAB5 CLIA Kit, 2410015H04Rik CLIA Kit, AI663973 CLIA Kit, AU021172 CLIA Kit, nnyRab5a CLIA Kit, DDBDRAFT_0168594 CLIA Kit, DDBDRAFT_0229401 CLIA Kit, DDB_0168594 CLIA Kit, DDB_0229401 CLIA Kit, rab5 CLIA Kit, RAB5A CLIA Kit, Rab5a CLIA Kit, rab5al CLIA Kit, zgc:56644 CLIA Kit, fj38g08 CLIA Kit, hm:zehn1144 CLIA Kit, rab5a CLIA Kit, wu:fj38g08 CLIA Kit, RAB5A, member RAS oncogene family CLIA Kit, rab5A protein CLIA Kit, Rab5a, GTPase CLIA Kit, predicted protein CLIA Kit, Rab GTPase CLIA Kit, RAB5A, member RAS oncogene family L homeolog CLIA Kit, RAB5A, member RAS oncogene family, b CLIA Kit, RAB5A, member RAS oncogene family, a CLIA Kit, RAB5A CLIA Kit, Rab5a CLIA Kit, rab5A CLIA Kit, rab5a CLIA Kit, rab5a.L CLIA Kit, rab5ab CLIA Kit, rab5aa CLIA Kit

Hintergrund:

Gene Name: RAB5A, Member RAS Oncogene Family

Gene Aliases: RAB5

Gen-ID: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity