RAB7A ELISA Kit

Produktnummer ABIN5658345

Produktdetails RAB7A ELISA Kit

Target: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of RAB7A, Member RAS Oncogene Family (RAB7A). No significant cross-reactivity or interference between RAB7A, Member RAS Oncogene Family (RAB7A) and analogues was observed.

Sensitivität: 0.113 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB7A, Member RAS Oncogene Family (RAB7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB7A, Member RAS Oncogene Family (RAB7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB7A, Member RAS Oncogene Family (RAB7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB7A, Member RAS Oncogene Family (RAB7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB7A, Member RAS Oncogene Family (RAB7A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB7A, Member RAS Oncogene Family (RAB7A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RAB7A

Target: RAB7A (RAB7A, Member RAS Oncogene Family (RAB7A))

Andere Bezeichnung: RAB7A, Member RAS Oncogene Family (RAB7A Produkte)

Synonyme: RAB5 ELISA Kit, PRO2706 ELISA Kit, RAB7 ELISA Kit, Rab7 ELISA Kit, rab7 ELISA Kit, pro2706 ELISA Kit, RAB7A ELISA Kit, LOC100231734 ELISA Kit, ARABIDOPSIS RAB GTPASE HOMOLOG G2 ELISA Kit, ATRAB7A ELISA Kit, ATRABG2 ELISA Kit, F7D8.20 ELISA Kit, F7D8_20 ELISA Kit, RAB GTPase homolog 7A ELISA Kit, DDBDRAFT_0190210 ELISA Kit, DDBDRAFT_0191507 ELISA Kit, DDB_0190210 ELISA Kit, DDB_0191507 ELISA Kit, Rab7a ELISA Kit, rab-7 ELISA Kit, zgc:64058 ELISA Kit, RAB5A, member RAS oncogene family ELISA Kit, RAB7A, member RAS oncogene family ELISA Kit, RAB7A, member RAS oncogene family L homeolog ELISA Kit, rab7A protein ELISA Kit, RAB GTPase homolog 7A ELISA Kit, Rab GTPase ELISA Kit, ras-related protein rab-7a ELISA Kit, RAB7, member RAS oncogene family ELISA Kit, RAB5A ELISA Kit, RAB7A ELISA Kit, Rab7a ELISA Kit, rab7a.L ELISA Kit, rab7a ELISA Kit, rab7A ELISA Kit, Rab7 ELISA Kit, rab7 ELISA Kit

Hintergrund:

Gene Name: RAB7A, Member RAS Oncogene Family

Gene Aliases: RAB7, Ras-related protein Rab-7a

Pathways: EGFR Signaling Pathway, Maintenance of Protein Location, SARS-CoV-2 Protein Interaktom