Fc epsilon RI/FCER1A ELISA Kit

Produktnummer ABIN5658372

Produktdetails Fc epsilon RI/FCER1A ELISA Kit

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Receptor I For The Fc Region Of Immunoglobulin E (FceRI). No significant cross-reactivity or interference between Receptor I For The Fc Region Of Immunoglobulin E (FceRI) and analogues was observed.

Sensitivität: 0.057 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin E (FceRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin E (FceRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin E (FceRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin E (FceRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin E (FceRI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin E (FceRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Fc epsilon RI/FCER1A

Target: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Andere Bezeichnung: Receptor I For The Fc Region Of Immunoglobulin E (FCER1A Produkte)

Synonyme: FCER1A ELISA Kit, FCE1A ELISA Kit, FcERI ELISA Kit, Iger01 ELISA Kit, RATIGER01 ELISA Kit, Fce1a ELISA Kit, Fcr-5 ELISA Kit, fcepsilonri ELISA Kit, Fc fragment of IgE receptor Ia ELISA Kit, Fc receptor, IgE, high affinity I, alpha polypeptide ELISA Kit, FCER1A ELISA Kit, Fcer1a ELISA Kit

Hintergrund:

Gene Name: Receptor I For The Fc Region Of Immunoglobulin E

Gene Aliases: FCER1A, FCE1A, FcER1 High affinity immunoglobulin epsilon receptor subunit alpha

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process