RGS6 ELISA Kit

Produktnummer ABIN5658419

Produktdetails RGS6 ELISA Kit

Target: RGS6 (Regulator of G-Protein Signaling 6 (RGS6))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Regulator Of G Protein Signaling 6 (RGS6). No significant cross-reactivity or interference between Regulator Of G Protein Signaling 6 (RGS6) and analogues was observed.

Sensitivität: 0.118 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulator Of G Protein Signaling 6 (RGS6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulator Of G Protein Signaling 6 (RGS6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulator Of G Protein Signaling 6 (RGS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regulator Of G Protein Signaling 6 (RGS6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulator Of G Protein Signaling 6 (RGS6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulator Of G Protein Signaling 6 (RGS6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RGS6

Target: RGS6 (Regulator of G-Protein Signaling 6 (RGS6))

Andere Bezeichnung: Regulator Of G Protein Signaling 6 (RGS6 Produkte)

Synonyme: RGS6 ELISA Kit, DKFZp459O0734 ELISA Kit, GAP ELISA Kit, hm:zeh0300 ELISA Kit, si:ch211-268e23.2 ELISA Kit, regulator of G protein signaling 6 ELISA Kit, regulator of G-protein signaling 6 ELISA Kit, regulator of G-protein signaling 6 S homeolog ELISA Kit, RGS6 ELISA Kit, Rgs6 ELISA Kit, rgs6.S ELISA Kit, rgs6 ELISA Kit

Hintergrund:

Gene Name: Regulator Of G Protein Signaling 6

Gene Aliases: GAP, S914

UniProt: F1LS67

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling