RETNLB ELISA Kit

Produktnummer ABIN5658455

Produktdetails RETNLB ELISA Kit

Target: RETNLB (Resistin Like beta (RETNLB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Resistin Like Beta (RETNLb). No significant cross-reactivity or interference between Resistin Like Beta (RETNLb) and analogues was observed.

Sensitivität: 22.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin Like Beta (RETNLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Resistin Like Beta (RETNLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Resistin Like Beta (RETNLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Resistin Like Beta (RETNLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Resistin Like Beta (RETNLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Andere Bezeichnung: Resistin Like Beta (RETNLB Produkte)

Synonyme: FIZZ1 ELISA Kit, FIZZ2 ELISA Kit, HXCP2 ELISA Kit, RELM-beta ELISA Kit, RELMb ELISA Kit, RELMbeta ELISA Kit, XCP2 ELISA Kit, RETNLB ELISA Kit, 9030012B21Rik ELISA Kit, Fizz2 ELISA Kit, Relmb ELISA Kit, Xcp3 ELISA Kit, resistin like beta ELISA Kit, RETNLB ELISA Kit, Retnlb ELISA Kit

Hintergrund:

Gene Name: Resistin Like Beta

Gene Aliases: XCP2, FIZZ2, HXCP2, CCRG, RETNL2, RELMb, Foundin In Flammatory Zone 2, Colon and small intestine-specific cysteine-rich protein, Colon carcinoma-related gene protein

Gen-ID: 84666

UniProt: Q9BQ08

Pathways: Hormone Activity