RGC32 ELISA Kit

Produktnummer ABIN5658457

Produktdetails RGC32 ELISA Kit

Target: RGC32 (C13orf15) (Chromosome 13 Open Reading Frame 15 (C13orf15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Response Gene To Complement 32 (RGC32). No significant cross-reactivity or interference between Response Gene To Complement 32 (RGC32) and analogues was observed.

Sensitivität: 0.128 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Response Gene To Complement 32 (RGC32). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Response Gene To Complement 32 (RGC32). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Response Gene To Complement 32 (RGC32), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Response Gene To Complement 32 (RGC32) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Response Gene To Complement 32 (RGC32) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RGC32

Target: RGC32 (C13orf15) (Chromosome 13 Open Reading Frame 15 (C13orf15))

Andere Bezeichnung: Response Gene To Complement 32 (C13orf15 Produkte)

Synonyme: C13orf15 ELISA Kit, RGC-32 ELISA Kit, RGC32 ELISA Kit, bA157L14.2 ELISA Kit, 1190002H23Rik ELISA Kit, Rgc-32 ELISA Kit, Rgc32 ELISA Kit, C12H13orf15 ELISA Kit, sb:cb658 ELISA Kit, wu:fa94a05 ELISA Kit, zgc:91870 ELISA Kit, regulator of cell cycle ELISA Kit, RGCC ELISA Kit, Rgcc ELISA Kit, rgcc ELISA Kit

Hintergrund:

Gene Name: Response Gene To Complement 32

Gene Aliases: RGCC, C13orf15, Regulator of cell cycle RGCC

Gen-ID: 28984

UniProt: Q9H4X1