ROCK2 ELISA Kit

Produktnummer ABIN5658485

Produktdetails ROCK2 ELISA Kit

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reaktivität: Human, Ratte, Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2). No significant cross-reactivity or interference between Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) and analogues was observed.

Sensitivität: 0.119 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Associated Coiled Coil Containing Protein Kinase 2 (Rock2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Andere Bezeichnung: Rho Associated Coiled Coil Containing Protein Kinase 2 (ROCK2 Produkte)

Synonyme: rocka-a ELISA Kit, xROK ELISA Kit, rock2 ELISA Kit, rok2 ELISA Kit, ROCK2 ELISA Kit, B230113H15Rik ELISA Kit, ROKalpha ELISA Kit, Rho-kinase ELISA Kit, Rock-II ELISA Kit, Rock2m ELISA Kit, mKIAA0619 ELISA Kit, ROCK-II ELISA Kit, ROK ELISA Kit, Rho-associated, coiled-coil containing protein kinase 2 L homeolog ELISA Kit, rho-associated, coiled-coil containing protein kinase 2a ELISA Kit, Rho associated coiled-coil containing protein kinase 2 ELISA Kit, Rho-associated, coiled-coil containing protein kinase 2 ELISA Kit, Rho-associated coiled-coil containing protein kinase 2 ELISA Kit, rock2.L ELISA Kit, rock2a ELISA Kit, ROCK2 ELISA Kit, Rock2 ELISA Kit

Hintergrund:

Gene Name: Rho Associated Coiled Coil Containing Protein Kinase 2

Pathways: Microtubule Dynamics, WNT Signalweg, Tube Formation