RRM1 ELISA Kit

Produktnummer ABIN5658511

Produktdetails RRM1 ELISA Kit

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ribonucleotide Reductase M1 (RRM1). No significant cross-reactivity or interference between Ribonucleotide Reductase M1 (RRM1) and analogues was observed.

Sensitivität: 0.115 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonucleotide Reductase M1 (RRM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonucleotide Reductase M1 (RRM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonucleotide Reductase M1 (RRM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonucleotide Reductase M1 (RRM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonucleotide Reductase M1 (RRM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonucleotide Reductase M1 (RRM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu RRM1

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Andere Bezeichnung: Ribonucleotide Reductase M1 (RRM1 Produkte)

Synonyme: CG5371 ELISA Kit, DmRNR1 ELISA Kit, Dmel\\CG5371 ELISA Kit, RNR1 ELISA Kit, RnrM1 ELISA Kit, RntL ELISA Kit, l(2)k06709 ELISA Kit, XRRM1 ELISA Kit, R1 ELISA Kit, RIR1 ELISA Kit, RR1 ELISA Kit, CHUNP6866 ELISA Kit, cb396 ELISA Kit, cb838 ELISA Kit, r1 ELISA Kit, sb:cb548 ELISA Kit, wu:fb39b07 ELISA Kit, wu:fi14b02 ELISA Kit, wu:fk95f07 ELISA Kit, ribonucleotide reductase catalytic subunit M1 ELISA Kit, Ribonucleoside diphosphate reductase large subunit ELISA Kit, ribonucleoside-diphosphate reductase large subunit ELISA Kit, ribonucleotide reductase M1 L homeolog ELISA Kit, ribonucleotide reductase M1 ELISA Kit, ribonucleotide reductase M1 polypeptide ELISA Kit, RRM1 ELISA Kit, RnrL ELISA Kit, LOC100472317 ELISA Kit, rrm1 ELISA Kit, rrm1.L ELISA Kit, Rrm1 ELISA Kit

Hintergrund:

Gene Name: Ribonucleotide Reductase M1

Gene Aliases: R1, RIR1, RR1, Ribonucleoside-diphosphate reductase large subunit

Gen-ID: 6240

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity