SPTLC1 ELISA Kit

Produktnummer ABIN5658719

Produktdetails SPTLC1 ELISA Kit

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). No significant cross-reactivity or interference between Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SPTLC1

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Andere Bezeichnung: Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1 Produkte)

Synonyme: ATSPT1 ELISA Kit, SERINE PALMITOYLTRANSFERASE 1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Afu6g00300 ELISA Kit, HSAN1 ELISA Kit, HSN1 ELISA Kit, LBC1 ELISA Kit, LCB1 ELISA Kit, SPT1 ELISA Kit, SPTI ELISA Kit, wu:fc75h04 ELISA Kit, wu:fp41c08 ELISA Kit, zgc:112247 ELISA Kit, AW552086 ELISA Kit, C77762 ELISA Kit, E030036H05 ELISA Kit, Lcb1 ELISA Kit, RGD1306617 ELISA Kit, Spt1 ELISA Kit, serine palmitoyltransferase 1 ELISA Kit, Serine palmitoyltransferase 1 ELISA Kit, serine palmitoyltransferase long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 ELISA Kit, serine palmitoyltransferase, long chain base subunit 1 L homeolog ELISA Kit, SPT1 ELISA Kit, AFUA_6G00300 ELISA Kit, PAS_FragB_0040 ELISA Kit, SPTLC1 ELISA Kit, sptlc1 ELISA Kit, sptlc1.L ELISA Kit, Sptlc1 ELISA Kit

Hintergrund:

Gene Name: Serine Palmitoyltransferase, Long Chain Base Subunit 1

Gene Aliases: HSAN, HSAN1, HSN1, LBC1, LCB1, SPT1, SPTI, Hereditary Sensory Neuropathy,Type 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1

Gen-ID: 268656

UniProt: O35704