SRSF2 ELISA Kit

Produktnummer ABIN5658728

Produktdetails SRSF2 ELISA Kit

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Serine/Arginine Rich Splicing Factor 2 (SRSF2). No significant cross-reactivity or interference between Serine/Arginine Rich Splicing Factor 2 (SRSF2) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Arginine Rich Splicing Factor 2 (SRSF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Arginine Rich Splicing Factor 2 (SRSF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Arginine Rich Splicing Factor 2 (SRSF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Arginine Rich Splicing Factor 2 (SRSF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Arginine Rich Splicing Factor 2 (SRSF2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Arginine Rich Splicing Factor 2 (SRSF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SRSF2

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Andere Bezeichnung: Serine/Arginine Rich Splicing Factor 2 (SRSF2 Produkte)

Synonyme: PR264 ELISA Kit, SC-35 ELISA Kit, SC35 ELISA Kit, SFRS2 ELISA Kit, SFRS2A ELISA Kit, SRp30b ELISA Kit, D11Wsu175e ELISA Kit, MRF-1 ELISA Kit, Pr264 ELISA Kit, Sfrs10 ELISA Kit, Sfrs2 ELISA Kit, pr264 ELISA Kit, sc-35 ELISA Kit, sc35 ELISA Kit, sfrs2 ELISA Kit, sfrs2a ELISA Kit, srp30b ELISA Kit, serine and arginine rich splicing factor 2 ELISA Kit, serine/arginine-rich splicing factor 2 ELISA Kit, serine/arginine-rich splicing factor 2 L homeolog ELISA Kit, SRSF2 ELISA Kit, Srsf2 ELISA Kit, srsf2.L ELISA Kit

Hintergrund:

Gene Name: Serine/Arginine Rich Splicing Factor 2

Gene Aliases: SFRS2, PR264, SC-35, SC35, SFRS2A, SRp30b, Splicing component, 35 kDa, Splicing factor, arginine/serine-rich 2

Gen-ID: 6427

UniProt: Q01130