LKB1 ELISA Kit

Produktnummer ABIN5658730

Produktdetails LKB1 ELISA Kit

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 11 (STK11). No significant cross-reactivity or interference between Serine/Threonine Kinase 11 (STK11) and analogues was observed.

Sensitivität: 0.051 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 11 (STK11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 11 (STK11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/Threonine Kinase 11 (STK11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/Threonine Kinase 11 (STK11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 11 (STK11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 11 (STK11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu LKB1

Target: LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

Andere Bezeichnung: Serine/Threonine Kinase 11 (STK11 Produkte)

Synonyme: pjs ELISA Kit, LKB1 ELISA Kit, XEEK1 ELISA Kit, Stk11 ELISA Kit, PJS ELISA Kit, hLKB1 ELISA Kit, AA408040 ELISA Kit, Lkb1 ELISA Kit, Par-4 ELISA Kit, R75140 ELISA Kit, mLKB1 ELISA Kit, wu:fj61a05 ELISA Kit, zgc:110180 ELISA Kit, xeek1 ELISA Kit, serine/threonine kinase 11 ELISA Kit, polarization-related protein LKB1 ELISA Kit, serine/threonine kinase 11 L homeolog ELISA Kit, STK11 ELISA Kit, stk11 ELISA Kit, LOC662493 ELISA Kit, Stk11 ELISA Kit, stk11.L ELISA Kit

Hintergrund:

Gene Name: Serine/Threonine Kinase 11

Gene Aliases: LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19

Gen-ID: 20869

UniProt: Q9WTK7

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt