STK39 CLIA Kit

Produktnummer ABIN5658732

Produktdetails STK39 CLIA Kit

Target: STK39 (serine threonine Kinase 39 (STK39))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Serine/Threonine Kinase 39 (STK39). No significant cross-reactivity or interference between Serine/Threonine Kinase 39 (STK39) and analogues was observed.

Sensitivität: 0.03 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Serine/Threonine Kinase 39 (STK39). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Serine/Threonine Kinase 39 (STK39). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Serine/Threonine Kinase 39 (STK39) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serine/Threonine Kinase 39 (STK39) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: STK39 (serine threonine Kinase 39 (STK39))

Andere Bezeichnung: Serine/Threonine Kinase 39 (STK39 Produkte)

Synonyme: dcht CLIA Kit, pask CLIA Kit, spak CLIA Kit, MGC69537 CLIA Kit, DCHT CLIA Kit, PASK CLIA Kit, SPAK CLIA Kit, Spak CLIA Kit, AW227544 CLIA Kit, AW556857 CLIA Kit, RF005 CLIA Kit, Rnl5 CLIA Kit, serine/threonine kinase 39 CLIA Kit, serine/threonine kinase 39 L homeolog CLIA Kit, serine threonine kinase 39 CLIA Kit, stk39 CLIA Kit, STK39 CLIA Kit, stk39.L CLIA Kit, Stk39 CLIA Kit

Hintergrund:

Gene Name: Serine/Threonine Kinase 39

Gene Aliases: DCHT, SPAK, STE20/SPS1-related proline-alanine-rich protein kinase

Gen-ID: 27347

UniProt: Q9UEW8