SMUG1 ELISA Kit

Produktnummer ABIN5658805

Produktdetails SMUG1 ELISA Kit

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). No significant cross-reactivity or interference between Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) and analogues was observed.

Sensitivität: 0.051 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Andere Bezeichnung: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1 Produkte)

Synonyme: SMUG1 ELISA Kit, xsmug1 ELISA Kit, 1200013B09Rik ELISA Kit, A930006H09Rik ELISA Kit, C85220 ELISA Kit, FDG ELISA Kit, HMUDG ELISA Kit, UNG3 ELISA Kit, single-strand-selective monofunctional uracil-DNA glycosylase 1 ELISA Kit, single-strand selective monofunctional uracil DNA glycosylase ELISA Kit, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog ELISA Kit, SMUG1 ELISA Kit, smug1 ELISA Kit, smuG1 ELISA Kit, smuG ELISA Kit, LOC5577584 ELISA Kit, CpipJ_CPIJ002767 ELISA Kit, Smug1 ELISA Kit, smug1.L ELISA Kit

Hintergrund:

Gene Name: Single Strand Selective Monofunctional Uracil DNA Glycosylase 1

Gene Aliases: FDG, HMUDG, UNG3

Gen-ID: 23583

UniProt: Q53HV7

Pathways: DNA Reparatur