SUMO2 ELISA Kit

Produktnummer ABIN5658841

Produktdetails SUMO2 ELISA Kit

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Small Ubiquitin Related Modifier Protein 2 (SUMO2). No significant cross-reactivity or interference between Small Ubiquitin Related Modifier Protein 2 (SUMO2) and analogues was observed.

Sensitivität: 0.126 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 2 (SUMO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 2 (SUMO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Ubiquitin Related Modifier Protein 2 (SUMO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 2 (SUMO2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Small Ubiquitin Related Modifier Protein 2 (SUMO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SUMO2

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Andere Bezeichnung: Small Ubiquitin Related Modifier Protein 2 (SUMO2 Produkte)

Synonyme: HSMT3 ELISA Kit, SMT3B ELISA Kit, SMT3H2 ELISA Kit, SUMO3 ELISA Kit, Smt3A ELISA Kit, SMT3A ELISA Kit, SMT3H1 ELISA Kit, SUMO-3 ELISA Kit, Smt3B ELISA Kit, Smt3h2 ELISA Kit, sumo2 ELISA Kit, zgc:92241 ELISA Kit, SUMO2 ELISA Kit, SUMO-2 ELISA Kit, Smt3b ELISA Kit, hsmt3 ELISA Kit, smt3b ELISA Kit, smt3h2 ELISA Kit, sumo-2 ELISA Kit, PSMT3 ELISA Kit, ATSUMO2 ELISA Kit, MCO15.11 ELISA Kit, MCO15_11 ELISA Kit, SMALL UBIQUITIN-LIKE MODIFIER 2 ELISA Kit, SUM2 ELISA Kit, SUMO 2 ELISA Kit, small ubiquitin-like modifier 2 ELISA Kit, small ubiquitin-like modifier 2 ELISA Kit, small ubiquitin-like modifier 3 ELISA Kit, small ubiquitin-like modifier 2b ELISA Kit, small ubiquitin-related modifier 2 ELISA Kit, small ubiquitin-like modifier 2 S homeolog ELISA Kit, small ubiquitin-like modifier 2 L homeolog ELISA Kit, SUMO2 ELISA Kit, SUMO3 ELISA Kit, Sumo2 ELISA Kit, sumo2b ELISA Kit, LOC714892 ELISA Kit, sumo2.S ELISA Kit, sumo2.L ELISA Kit

Hintergrund:

Gene Name: Small Ubiquitin Related Modifier Protein 2

Gene Aliases: HSMT3, SMT3B, SMT3H2, SUMO-3, Sentrin-2, Ubiquitin-like protein SMT3B

Gen-ID: 6613

UniProt: P61956

Pathways: Methionine Biosynthetic Process