SNAIL ELISA Kit

Produktnummer ABIN5658844

Produktdetails SNAIL ELISA Kit

Target: SNAIL (SNAI1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Snail Homolog 1 (SNAI1). No significant cross-reactivity or interference between Snail Homolog 1 (SNAI1) and analogues was observed.

Sensitivität: 0.063 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Snail Homolog 1 (SNAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Snail Homolog 1 (SNAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Snail Homolog 1 (SNAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Snail Homolog 1 (SNAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Snail Homolog 1 (SNAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SNAIL

Target: SNAIL (SNAI1)

Andere Bezeichnung: Snail Homolog 1 (SNAI1 Produkte)

Synonyme: SLUGH2 ELISA Kit, SNA ELISA Kit, SNAH ELISA Kit, SNAIL ELISA Kit, SNAIL1 ELISA Kit, dJ710H13.1 ELISA Kit, AI194338 ELISA Kit, Sna ELISA Kit, Sna1 ELISA Kit, Snail ELISA Kit, Snail1 ELISA Kit, SNAI1 ELISA Kit, BG:DS01845.1 ELISA Kit, CG3956 ELISA Kit, Dmel\\CG3956 ELISA Kit, Sco ELISA Kit, br28 ELISA Kit, br29 ELISA Kit, l(2)35Db ELISA Kit, l(2)br28 ELISA Kit, l(2)br29 ELISA Kit, l(3)br28 ELISA Kit, l35Db ELISA Kit, sn ELISA Kit, Xsnail ELISA Kit, sna ELISA Kit, snail1 ELISA Kit, xSna ELISA Kit, Snai ELISA Kit, sna2 ELISA Kit, snail2 ELISA Kit, cb643 ELISA Kit, sna-1 ELISA Kit, sna1 ELISA Kit, snai1 ELISA Kit, snail1a ELISA Kit, zgc:109820 ELISA Kit, snail family transcriptional repressor 1 ELISA Kit, snail family zinc finger 1 ELISA Kit, snail homolog 1 (Drosophila) ELISA Kit, snail ELISA Kit, zinc-finger transcription factor Snail ELISA Kit, snail family zinc finger 1 S homeolog ELISA Kit, protein escargot ELISA Kit, transcription factor protein ELISA Kit, snail zinc finger protein ELISA Kit, snail family zinc finger 1b ELISA Kit, snail family zinc finger 1a ELISA Kit, SNAI1 ELISA Kit, Snai1 ELISA Kit, sna ELISA Kit, snail ELISA Kit, snai1.S ELISA Kit, LOC410464 ELISA Kit, snai1b ELISA Kit, snai1a ELISA Kit

Hintergrund:

Gene Name: Snail Homolog 1

Gene Aliases: SLUGH2, SNA, SNAH, Snail 1 Zinc Finger Protein

Gen-ID: 6615

UniProt: O95863

Pathways: Negative Regulation of intrinsic apoptotic Signaling