S1PR1 ELISA Kit

Produktnummer ABIN5658914

Produktdetails S1PR1 ELISA Kit

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate Receptor 1 (S1PR1). No significant cross-reactivity or interference between Sphingosine 1 Phosphate Receptor 1 (S1PR1) and analogues was observed.

Sensitivität: 0.121 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 1 (S1PR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 1 (S1PR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 1 (S1PR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 1 (S1PR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 1 (S1PR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 1 (S1PR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu S1PR1

Target: S1PR1 (Sphingosine-1-Phosphate Receptor 1 (S1PR1))

Andere Bezeichnung: Sphingosine 1 Phosphate Receptor 1 (S1PR1 Produkte)

Synonyme: CD363 ELISA Kit, CHEDG1 ELISA Kit, D1S3362 ELISA Kit, ECGF1 ELISA Kit, EDG-1 ELISA Kit, EDG1 ELISA Kit, S1P1 ELISA Kit, AI849002 ELISA Kit, Edg1 ELISA Kit, Lpb1 ELISA Kit, S1p ELISA Kit, S1p1 ELISA Kit, S1P receptor 1 ELISA Kit, S1P receptor Edg-1 ELISA Kit, edg1 ELISA Kit, etID43307.22 ELISA Kit, s1p1 ELISA Kit, wu:fb98f05 ELISA Kit, s1pr1 ELISA Kit, xts1p1 ELISA Kit, sphingosine-1-phosphate receptor 1 ELISA Kit, sphingosine-1-phosphate receptor 1 L homeolog ELISA Kit, S1PR1 ELISA Kit, S1pr1 ELISA Kit, s1pr1 ELISA Kit, s1pr1.L ELISA Kit

Hintergrund:

Gene Name: Sphingosine 1 Phosphate Receptor 1

Gene Aliases: CD363, EDG1, S1-PR1, S1P1, ECGF1, CHEDG1, Endothelial differentiation G-protein coupled receptor 1, Sphingolipid G-Protein-Coupled Receptor 1

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2