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SCCA1 ELISA Kit

SCCA1 Reaktivität: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5658933
  • Target Alle SCCA1 ELISA Kits anzeigen
    SCCA1 (Squamous Cell Carcinoma Antigen 1 (SCCA1))
    Reaktivität
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.12 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.12 pg/mL
    Applikation
    ELISA
    Proben
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Squamous Cell Carcinoma Antigen 1 (SCCA1). No significant cross-reactivity or interference between Squamous Cell Carcinoma Antigen 1 (SCCA1) and analogues was observed.
    Sensitivität
    27 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Squamous Cell Carcinoma Antigen 1 (SCCA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Squamous Cell Carcinoma Antigen 1 (SCCA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Squamous Cell Carcinoma Antigen 1 (SCCA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Squamous Cell Carcinoma Antigen 1 (SCCA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 1 (SCCA1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Squamous Cell Carcinoma Antigen 1 (SCCA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle SCCA1 ELISA Kits anzeigen
    SCCA1 (Squamous Cell Carcinoma Antigen 1 (SCCA1))
    Andere Bezeichnung
    Squamous Cell Carcinoma Antigen 1 (SCCA1 Produkte)
    Synonyme
    HsT1196 ELISA Kit, SCC ELISA Kit, SCCA-1 ELISA Kit, SCCA-PD ELISA Kit, SCCA1 ELISA Kit, T4-A ELISA Kit, LEUPIN ELISA Kit, PI11 ELISA Kit, SCCA-2 ELISA Kit, SCCA2 ELISA Kit, serpin family B member 3 ELISA Kit, serpin family B member 4 ELISA Kit, SERPINB3 ELISA Kit, SERPINB4 ELISA Kit
    Hintergrund

    Gene Name: Squamous Cell Carcinoma Antigen 1

    Gene Aliases: SERPINB3, T4-A, Serpin Peptidase Inhibitor,Clade B(ovalbumin),Member 3, Serpin B3

    Gen-ID
    6317
    UniProt
    P29508
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