SOD3 ELISA Kit

Produktnummer ABIN5659006

Produktdetails SOD3 ELISA Kit

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125 pg/mL - 8000 pg/mL

Untere Nachweisgrenze: 125 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 3, Extracellular (SOD3). No significant cross-reactivity or interference between Superoxide Dismutase 3, Extracellular (SOD3) and analogues was observed.

Sensitivität: 55 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 3, Extracellular (SOD3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 3, Extracellular (SOD3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 3, Extracellular (SOD3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 3, Extracellular (SOD3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Andere Bezeichnung: Superoxide Dismutase 3, Extracellular (SOD3 Produkte)

Synonyme: EC-SOD ELISA Kit, ECSOD ELISA Kit, SOD3 ELISA Kit, AI314465 ELISA Kit, LOC780439 ELISA Kit, fb05f10 ELISA Kit, si:dkey-117m1.6 ELISA Kit, sod3 ELISA Kit, wu:fb05f10 ELISA Kit, ECSODPT ELISA Kit, CuZnSOD ELISA Kit, SODA.4 ELISA Kit, superoxide dismutase 3 ELISA Kit, superoxide dismutase 3, extracellular ELISA Kit, superoxide dismutase 3, extracellular b ELISA Kit, superoxide dismutase 1 ELISA Kit, superoxide dismutase precursor ,putative ELISA Kit, SOD3 ELISA Kit, Sod3 ELISA Kit, sod3b ELISA Kit, sod3 ELISA Kit, Sod1 ELISA Kit, Smp_095980 ELISA Kit

Hintergrund:

Gene Name: Superoxide Dismutase 3, Extracellular

Gene Aliases: SOD-3, EC-SOD, Extracellular Superoxide Dismutase Cu-Zn

Gen-ID: 6649

UniProt: P08294