SOCS2 ELISA Kit

Produktnummer ABIN5659018

Produktdetails SOCS2 ELISA Kit

Target: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Suppressors Of Cytokine Signaling 2 (SOCS2). No significant cross-reactivity or interference between Suppressors Of Cytokine Signaling 2 (SOCS2) and analogues was observed.

Sensitivität: 0.124 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressors Of Cytokine Signaling 2 (SOCS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressors Of Cytokine Signaling 2 (SOCS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressors Of Cytokine Signaling 2 (SOCS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressors Of Cytokine Signaling 2 (SOCS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppressors Of Cytokine Signaling 2 (SOCS2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppressors Of Cytokine Signaling 2 (SOCS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SOCS2

Target: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Andere Bezeichnung: Suppressors Of Cytokine Signaling 2 (SOCS2 Produkte)

Synonyme: CIS2 ELISA Kit, Cish2 ELISA Kit, SOCS-2 ELISA Kit, SSI-2 ELISA Kit, SSI2 ELISA Kit, STATI2 ELISA Kit, Cish1 ELISA Kit, Socs-1 ELISA Kit, Socs-2 ELISA Kit, si:dkey-202b22.1 ELISA Kit, socs2 ELISA Kit, cis2 ELISA Kit, ssi2 ELISA Kit, cish2 ELISA Kit, ssi-2 ELISA Kit, socs-2 ELISA Kit, stati2 ELISA Kit, 8030460M17 ELISA Kit, AI527257 ELISA Kit, AW108012 ELISA Kit, D130043N08Rik ELISA Kit, JAB ELISA Kit, hg ELISA Kit, Cradd ELISA Kit, Socs2 ELISA Kit, suppressor of cytokine signaling 2 ELISA Kit, suppressor of cytokine signaling 1 ELISA Kit, death domain-containing protein CRADD ELISA Kit, suppressor of cytokine signaling 2 S homeolog ELISA Kit, SOCS2 ELISA Kit, Socs1 ELISA Kit, Socs2 ELISA Kit, socs2 ELISA Kit, LOC100716104 ELISA Kit, socs2.S ELISA Kit

Hintergrund:

Gene Name: Suppressors Of Cytokine Signaling 2

Gene Aliases: CIS2, Cish2, SOCS-2, SSI-2, SSI2, STATI2, Cytokine-inducible SH2 protein 2, STAT-induced STAT inhibitor 2

Gen-ID: 84607

UniProt: O88582

Pathways: JAK-STAT Signalweg, Response to Growth Hormone Stimulus