T-Bet ELISA Kit

Produktnummer ABIN5659077

Produktdetails T-Bet ELISA Kit

Target: T-Bet

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of T-Box Protein 21 (TBX21). No significant cross-reactivity or interference between T-Box Protein 21 (TBX21) and analogues was observed.

Sensitivität: 0.052 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21 (TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21 (TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21 (TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Box Protein 21 (TBX21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Box Protein 21 (TBX21) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Box Protein 21 (TBX21) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu T-Bet

Target: T-Bet

Andere Bezeichnung: T-Box Protein 21 (T-Bet Produkte)

Synonyme: T-PET ELISA Kit, T-bet ELISA Kit, TBET ELISA Kit, TBLYM ELISA Kit, TBT1 ELISA Kit, Tbet ELISA Kit, Tblym ELISA Kit, T-box 21 ELISA Kit, TBX21 ELISA Kit, Tbx21 ELISA Kit

Hintergrund:

Gene Name: T-Box Protein 21

Gene Aliases: TBLYM, TBET, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet

Gen-ID: 30009

UniProt: Q9UL17