TIMD4 ELISA Kit

Produktnummer ABIN5659079

Produktdetails TIMD4 ELISA Kit

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4). No significant cross-reactivity or interference between T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TIMD4

Target: TIMD4 (T-Cell Immunoglobulin and Mucin Domain Containing 4 (TIMD4))

Andere Bezeichnung: T-Cell Immunoglobulin And Mucin Domain Containing Protein 4 (TIMD4 Produkte)

Synonyme: RGD1564516 ELISA Kit, MGC99322 ELISA Kit, TIMD4 ELISA Kit, B430010N18Rik ELISA Kit, TIM-4 ELISA Kit, Tim4 ELISA Kit, SMUCKLER ELISA Kit, TIM4 ELISA Kit, T-cell immunoglobulin and mucin domain containing 4 ELISA Kit, T-cell immunoglobulin and mucin domain containing 4 L homeolog ELISA Kit, T cell immunoglobulin and mucin domain containing 4 ELISA Kit, Timd4 ELISA Kit, TIMD4 ELISA Kit, timd4.L ELISA Kit

Hintergrund:

Gene Name: T-Cell Immunoglobulin And Mucin Domain Containing Protein 4

Gene Aliases: TIM4, T-cell membrane protein 4, T-cell immunoglobulin mucin receptor 4

Pathways: Cancer Immune Checkpoints