CD81 ELISA Kit

Produktnummer ABIN5659093

Produktdetails CD81 ELISA Kit

Target: CD81

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Target Of The Antiproliferative Antibody 1 (TAPA1). No significant cross-reactivity or interference between Target Of The Antiproliferative Antibody 1 (TAPA1) and analogues was observed.

Sensitivität: 0.115 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Target Of The Antiproliferative Antibody 1 (TAPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Target Of The Antiproliferative Antibody 1 (TAPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Target Of The Antiproliferative Antibody 1 (TAPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Target Of The Antiproliferative Antibody 1 (TAPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Target Of The Antiproliferative Antibody 1 (TAPA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Target Of The Antiproliferative Antibody 1 (TAPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CD81

Target: CD81

Andere Bezeichnung: Target Of The Antiproliferative Antibody 1 (CD81 Produkte)

Synonyme: Tapa-1 ELISA Kit, Tapa1 ELISA Kit, Tspan28 ELISA Kit, CVID6 ELISA Kit, S5.7 ELISA Kit, TAPA1 ELISA Kit, TSPAN28 ELISA Kit, CD81 molecule ELISA Kit, CD81 molecule b ELISA Kit, CD81 antigen ELISA Kit, Cd81 molecule ELISA Kit, CD81 ELISA Kit, cd81b ELISA Kit, Cd81 ELISA Kit

Hintergrund:

Gene Name: Target Of The Antiproliferative Antibody 1

Gene Aliases: CD81, S5.7, TAPA1, TSPAN28, Tetraspanin 28, Target Of Antiproliferative Antibody 1, 26 kDa cell surface protein TAPA-1

Gen-ID: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C