CD63 CLIA Kit

Produktnummer ABIN5659139

Produktdetails CD63 CLIA Kit

Target: CD63

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tetraspanin 30 (TSPAN30). No significant cross-reactivity or interference between Tetraspanin 30 (TSPAN30) and analogues was observed.

Sensitivität: 5.01 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30 (TSPAN30). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tetraspanin 30 (TSPAN30). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tetraspanin 30 (TSPAN30) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tetraspanin 30 (TSPAN30) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tetraspanin 30 (TSPAN30) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: CD63

Andere Bezeichnung: Tetraspanin 30 (CD63 Produkte)

Synonyme: C75951 CLIA Kit, ME491 CLIA Kit, Tspan30 CLIA Kit, LAMP-3 CLIA Kit, MLA1 CLIA Kit, OMA81H CLIA Kit, TSPAN30 CLIA Kit, TSP29FA CLIA Kit, CD63 antigen CLIA Kit, CD63 molecule CLIA Kit, Cd63 molecule CLIA Kit, Cd63 CLIA Kit, CD63 CLIA Kit

Hintergrund:

Gene Name: Tetraspanin 30

Gene Aliases: CD63, LAMP-3, ME491, MLA1, OMA81H, Melanoma 1 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Ocular melanoma-associated antigen

Gen-ID: 967

UniProt: P08962