TXNRD1 CLIA Kit

Produktnummer ABIN5659154

Produktdetails TXNRD1 CLIA Kit

Target: TXNRD1 (Thioredoxin Reductase 1 (TXNRD1))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 468.75 pg/mL - 30000 pg/mL

Untere Nachweisgrenze: 468.75 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Thioredoxin Reductase 1 (TrxR1). No significant cross-reactivity or interference between Thioredoxin Reductase 1 (TrxR1) and analogues was observed.

Sensitivität: 15.05 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Reductase 1 (TrxR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thioredoxin Reductase 1 (TrxR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thioredoxin Reductase 1 (TrxR1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TrxR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TrxR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: TXNRD1 (Thioredoxin Reductase 1 (TXNRD1))

Andere Bezeichnung: Thioredoxin Reductase 1 (TXNRD1 Produkte)

Synonyme: TrxR1 CLIA Kit, cb682 CLIA Kit, fb83a08 CLIA Kit, wu:fb83a08 CLIA Kit, GB14972 CLIA Kit, MGC85342 CLIA Kit, NADPH-dependent thioredoxin reductase C CLIA Kit, T32G6.20 CLIA Kit, T32G6_20 CLIA Kit, GRIM-12 CLIA Kit, TR CLIA Kit, TR1 CLIA Kit, TRXR1 CLIA Kit, TXNR CLIA Kit, Tr CLIA Kit, thioredoxin reductase 1 CLIA Kit, thioredoxin reductase 1 L homeolog CLIA Kit, NADPH-dependent thioredoxin reductase C CLIA Kit, ThioRedoXin Reductase CLIA Kit, TXNRD1 CLIA Kit, txnrd1 CLIA Kit, Trxr-1 CLIA Kit, txnrd1.L CLIA Kit, NTRC CLIA Kit, trxr-1 CLIA Kit, Txnrd1 CLIA Kit

Hintergrund:

Gene Name: Thioredoxin Reductase 1

Gene Aliases: TXNRD1, TR1, GRIM12, KDRF, TRXR-1, TXNR, Gene associated with retinoic and interferon-induced mortality 12 protein, KM-102-derived reductase-like factor

Pathways: Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Cell RedoxHomeostasis