TRAF6 ELISA Kit

Produktnummer ABIN5659338

Produktdetails TRAF6 ELISA Kit

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of TNF Receptor Associated Factor 6 (TRAF6). No significant cross-reactivity or interference between TNF Receptor Associated Factor 6 (TRAF6) and analogues was observed.

Sensitivität: 0.053 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 6 (TRAF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNF Receptor Associated Factor 6 (TRAF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TRAF6

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Andere Bezeichnung: TNF Receptor Associated Factor 6 (TRAF6 Produkte)

Synonyme: MGC:3310 ELISA Kit, RNF85 ELISA Kit, 2310003F17Rik ELISA Kit, AI851288 ELISA Kit, C630032O20Rik ELISA Kit, rnf85 ELISA Kit, traf6 ELISA Kit, xtraf6 ELISA Kit, CG10961 ELISA Kit, DTRAF2 ELISA Kit, Dmel\\CG10961 ELISA Kit, TRAF ELISA Kit, TRAF2 ELISA Kit, TRAF6 ELISA Kit, Traf2 ELISA Kit, dTRAF2 ELISA Kit, dTRAF6 ELISA Kit, dTraf2 ELISA Kit, dtraf2 ELISA Kit, GB10539 ELISA Kit, fi15f02 ELISA Kit, wu:fi15f02 ELISA Kit, zgc:63704 ELISA Kit, TNF receptor associated factor 6 ELISA Kit, TNF receptor-associated factor 6 ELISA Kit, TNF receptor associated factor 6 L homeolog ELISA Kit, TNF-receptor-associated factor 6 ELISA Kit, TNF receptor associated factor 6 S homeolog ELISA Kit, TRAF6 ELISA Kit, Traf6 ELISA Kit, traf6.L ELISA Kit, traf6 ELISA Kit, LOC551819 ELISA Kit, traf6.S ELISA Kit

Hintergrund:

Gene Name: TNF Receptor Associated Factor 6

Gene Aliases: RNF85, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer

Gen-ID: 7189

UniProt: Q9Y4K3

Pathways: NF-kappaB Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway