TLR3 CLIA Kit
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- Target Alle TLR3 CLIA Kits anzeigen
- TLR3 (Toll-Like Receptor 3 (TLR3))
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Reaktivität
- Maus
- Nachweismethode
- Chemiluminescent
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 62.5 pg/mL - 4000 pg/mL
- Untere Nachweisgrenze
- 62.5 pg/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 3 (TLR3). No significant cross-reactivity or interference between Toll Like Receptor 3 (TLR3) and analogues was observed.
- Sensitivität
- 1.93 pg/mL
- Top Product
- Discover our top product TLR3 ELISA Kit
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 2 - 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The microplate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 3 (TLR3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Toll Like Receptor 3 (TLR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Toll Like Receptor 3 (TLR3) level in the sample or standard.,
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 3 (TLR3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 3 (TLR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle TLR3 CLIA Kits anzeigen
- TLR3 (Toll-Like Receptor 3 (TLR3))
- Andere Bezeichnung
- Toll Like Receptor 3 (TLR3 Produkte)
- Synonyme
- CD283 CLIA Kit, IIAE2 CLIA Kit, AI957183 CLIA Kit, zgc:123006 CLIA Kit, toll like receptor 3 CLIA Kit, toll-like receptor 3 CLIA Kit, TLR3 CLIA Kit, Tlr3 CLIA Kit, tlr3 CLIA Kit
- Hintergrund
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Gene Name: Toll Like Receptor 3
Gene Aliases: CD283
- Gen-ID
- 142980
- UniProt
- Q99MB1
- Pathways
- TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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