TLR5 ELISA Kit

Produktnummer ABIN5659361

Produktdetails TLR5 ELISA Kit

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 5 (TLR5). No significant cross-reactivity or interference between Toll Like Receptor 5 (TLR5) and analogues was observed.

Sensitivität: 0.32 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 5 (TLR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor 5 (TLR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 5 (TLR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Toll Like Receptor 5 (TLR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 5 (TLR5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 5 (TLR5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TLR5

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Andere Bezeichnung: Toll Like Receptor 5 (TLR5 Produkte)

Synonyme: SLEB1 ELISA Kit, TIL3 ELISA Kit, TLR-5 ELISA Kit, sleb1 ELISA Kit, til3 ELISA Kit, tlr-5 ELISA Kit, tlr5 ELISA Kit, toll like receptor 5 ELISA Kit, toll-like receptor 5 ELISA Kit, toll like receptor 5 L homeolog ELISA Kit, toll-like receptor 5b ELISA Kit, TLR5 ELISA Kit, Tlr5 ELISA Kit, tlr5.L ELISA Kit, tlr-5 ELISA Kit, tlr5b ELISA Kit

Hintergrund:

Gene Name: Toll Like Receptor 5

Gene Aliases: CD285, SLEB1, TIL3, Toll/Interleukin-1 Receptor-Like Protein 3

UniProt: O60602

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades