TNNC1 ELISA Kit

Produktnummer ABIN5659552

Produktdetails TNNC1 ELISA Kit

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Troponin C Type 1, Slow (TNNC1). No significant cross-reactivity or interference between Troponin C Type 1, Slow (TNNC1) and analogues was observed.

Sensitivität: 27.3 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin C Type 1, Slow (TNNC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin C Type 1, Slow (TNNC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin C Type 1, Slow (TNNC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin C Type 1, Slow (TNNC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin C Type 1, Slow (TNNC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin C Type 1, Slow (TNNC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TNNC1

Target: TNNC1 (Cardiac Troponin C (TNNC1))

Andere Bezeichnung: Troponin C Type 1, Slow (TNNC1 Produkte)

Synonyme: CMD1Z ELISA Kit, CMH13 ELISA Kit, TN-C ELISA Kit, TNC ELISA Kit, TNNC ELISA Kit, cTnC ELISA Kit, tnnc1 ELISA Kit, zgc:103465 ELISA Kit, PCTNC1 ELISA Kit, cmd1z ELISA Kit, cmh13 ELISA Kit, tnc ELISA Kit, tnnc ELISA Kit, MGC80066 ELISA Kit, TNNC1 ELISA Kit, AI874626 ELISA Kit, TnC ELISA Kit, cTnI ELISA Kit, tncc ELISA Kit, Tncc ELISA Kit, stnnc ELISA Kit, zgc:86932 ELISA Kit, troponin C1, slow skeletal and cardiac type ELISA Kit, troponin C type 1a (slow) ELISA Kit, troponin C type 1 (slow) L homeolog ELISA Kit, cardiac troponin C ELISA Kit, troponin C, cardiac/slow skeletal ELISA Kit, troponin C type 1b (slow) ELISA Kit, TNNC1 ELISA Kit, tnnc1a ELISA Kit, tnnc1.L ELISA Kit, tnnc1 ELISA Kit, Tnnc1 ELISA Kit, tnnc1b ELISA Kit

Hintergrund:

Gene Name: Troponin C Type 1, Slow

Gene Aliases: TNC, TroponinC, Cardiac, Troponin C, slow skeletal and cardiac muscles

Gen-ID: 7134

UniProt: P63316