TPT1 CLIA Kit

Produktnummer ABIN5659697

Produktdetails TPT1 CLIA Kit

Target: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 312.5 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312.5 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1). No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.

Sensitivität: 10.33 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tumor Protein, Translationally Controlled 1 (TPT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tumor Protein, Translationally Controlled 1 (TPT1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein, Translationally Controlled 1 (TPT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: TPT1 (Tumor Protein, Translationally-Controlled 1 (TPT1))

Andere Bezeichnung: Tumor Protein, Translationally Controlled 1 (TPT1 Produkte)

Synonyme: HRF CLIA Kit, TCTP CLIA Kit, p02 CLIA Kit, Trt CLIA Kit, p21 CLIA Kit, p23 CLIA Kit, TPT1 CLIA Kit, TCT1 CLIA Kit, tctp CLIA Kit, fa28a02 CLIA Kit, id:ibd2078 CLIA Kit, wu:fa28a02 CLIA Kit, PCHK23 CLIA Kit, hrf CLIA Kit, MGC79720 CLIA Kit, Tpt1 CLIA Kit, translationally controlled tumor protein CLIA Kit, CG4800 CLIA Kit, Dmel\\CG4800 CLIA Kit, dTCTP CLIA Kit, Tctp CLIA Kit, tumor protein, translationally-controlled 1 CLIA Kit, tumor protein, translationally-controlled 1 L homeolog CLIA Kit, translationally-controlled tumor protein CLIA Kit, translationally controlled tumor protein CLIA Kit, translationally controlled tumor-like protein CLIA Kit, Translationally controlled tumor protein CLIA Kit, translationally-controlled tumor protein homolog CLIA Kit, P23 tumor protein-like CLIA Kit, TPT1 CLIA Kit, Tpt1 CLIA Kit, tpt1 CLIA Kit, tpt1.L CLIA Kit, LOC711798 CLIA Kit, TCTP CLIA Kit, LOC548038 CLIA Kit, Tctp CLIA Kit, LOC108708523 CLIA Kit, LOC109404863 CLIA Kit, LOC105389397 CLIA Kit, LOC102577554 CLIA Kit

Hintergrund:

Gene Name: Tumor Protein, Translationally Controlled 1

Gene Aliases: HRF, TCTP, p02, p23, Fortilin, Translationally-Controlled Tumor Protein, Histamine-releasing factor

Gen-ID: 22070

UniProt: P63028

Pathways: Response to Water Deprivation, Regulation of Cell Size, Negative Regulation of intrinsic apoptotic Signaling