UBE2L3 CLIA Kit

Produktnummer ABIN5659736

Produktdetails UBE2L3 CLIA Kit

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). No significant cross-reactivity or interference between Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) and analogues was observed.

Sensitivität: 5.28 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2L3 (UBE2L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Conjugating Enzyme E2L3 (UBE2L3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Andere Bezeichnung: Ubiquitin Conjugating Enzyme E2L3 (UBE2L3 Produkte)

Synonyme: E2-F1 CLIA Kit, L-UBC CLIA Kit, UBCH7 CLIA Kit, UbcM4 CLIA Kit, C79827 CLIA Kit, Ubce7 CLIA Kit, ube2l3 CLIA Kit, wu:fc22h11 CLIA Kit, zgc:86727 CLIA Kit, UBE2L3 CLIA Kit, ube2l3l CLIA Kit, wu:fb38h03 CLIA Kit, ubiquitin conjugating enzyme E2 L3 CLIA Kit, ubiquitin-conjugating enzyme E2L 3 CLIA Kit, ubiquitin-conjugating enzyme E2L 3a CLIA Kit, ubiquitin conjugating enzyme E2 L3 S homeolog CLIA Kit, ubiquitin-conjugating enzyme E2L 3b CLIA Kit, UBE2L3 CLIA Kit, Ube2l3 CLIA Kit, ube2l3a CLIA Kit, ube2l3.S CLIA Kit, ube2l3b CLIA Kit

Hintergrund:

Gene Name: Ubiquitin Conjugating Enzyme E2L3

Gene Aliases: L-UBC, E2-F1, UBCH7, UbcM4, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3

Gen-ID: 7332

UniProt: P68036