UBR4 ELISA Kit

Produktnummer ABIN5659742

Produktdetails UBR4 ELISA Kit

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). No significant cross-reactivity or interference between Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) and analogues was observed.

Sensitivität: 0.064 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu UBR4

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Andere Bezeichnung: Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4 Produkte)

Synonyme: ZUBR1 ELISA Kit, RBAF600 ELISA Kit, p600 ELISA Kit, 1810009A16Rik ELISA Kit, A930005E13Rik ELISA Kit, D930005K06Rik ELISA Kit, Gm1032 ELISA Kit, Gm1666 ELISA Kit, N28143 ELISA Kit, Zubr1 ELISA Kit, mKIAA0462 ELISA Kit, Rbaf600 ELISA Kit, ubiquitin protein ligase E3 component n-recognin 4 ELISA Kit, E3 ubiquitin-protein ligase UBR4 ELISA Kit, UBR4 ELISA Kit, Ubr4 ELISA Kit, LOC100511934 ELISA Kit

Hintergrund:

Gene Name: Ubiquitin Protein Ligase E3 Component N-Recognin 4

Gene Aliases: RBAF600, p600, ZUBR1, Zinc Finger,UBR1 Type 1, N-recognin-4, 600 kDa retinoblastoma protein-associated factor, Retinoblastoma-associated factor of 600 kDa

Gen-ID: 23352

UniProt: Q5T4S7