UCP2 ELISA Kit

Produktnummer ABIN5659759

Produktdetails UCP2 ELISA Kit

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Uncoupling Protein 2, Mitochondrial (UCP2). No significant cross-reactivity or interference between Uncoupling Protein 2, Mitochondrial (UCP2) and analogues was observed.

Sensitivität: 0.051 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uncoupling Protein 2, Mitochondrial (UCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uncoupling Protein 2, Mitochondrial (UCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uncoupling Protein 2, Mitochondrial (UCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uncoupling Protein 2, Mitochondrial (UCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uncoupling Protein 2, Mitochondrial (UCP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uncoupling Protein 2, Mitochondrial (UCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Andere Bezeichnung: Uncoupling Protein 2, Mitochondrial (UCP2 Produkte)

Synonyme: UCP2 ELISA Kit, DKFZp470P1633 ELISA Kit, ucp2 ELISA Kit, MGC53319 ELISA Kit, MGC75881 ELISA Kit, cb74 ELISA Kit, ATUCP2 ELISA Kit, K19M22.21 ELISA Kit, K19M22_21 ELISA Kit, uncoupling protein 2 ELISA Kit, BMIQ4 ELISA Kit, SLC25A8 ELISA Kit, UCPH ELISA Kit, Slc25a8 ELISA Kit, mitochondrial uncoupling protein 2 ELISA Kit, uncoupling protein 2 ELISA Kit, uncoupling protein 2 (mitochondrial, proton carrier) L homeolog ELISA Kit, uncoupling protein 2 (mitochondrial, proton carrier) ELISA Kit, uncoupling protein 1 ELISA Kit, PTRG_09289 ELISA Kit, UCP2 ELISA Kit, LOC100282746 ELISA Kit, ucp2.L ELISA Kit, ucp2 ELISA Kit, ucp1 ELISA Kit, Ucp2 ELISA Kit

Hintergrund:

Gene Name: Uncoupling Protein 2, Mitochondrial

Gene Aliases: SLC25A8, UCPH, Solute carrier family 25 member 8

Gen-ID: 7351

UniProt: P55851

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport